Researchers have made artificial mouse embryos from stem cells—no sperm or eggs required—and used an innovative bioreactor to nurture their creations for longer than any previous embryo models.
What happens in embryonic development is one of nature’s best guarded secrets, unfolding deep in the mother’s body. Now, researchers have opened a new window on the process. They’ve made artificial mouse embryos from stem cells—no sperm or eggs required—and used an innovative bioreactor to nurture their creations for longer than any previous embryo models. The simulated embryos developed anatomy that matched the real thing and “very impressive similarities at the cellular level.
One obstacle has been keeping the ersatz embryos alive for more than a few days. Last year, Hanna and colleaguesthat allowed them to grow standard mouse embryos outside of the mother’s body for a record 11 days. A key step involves placing the embryos in an incubator outfitted with a Ferris wheel–like device, which rotates the embryos inside bottles of liquid filled with nutrients and growth factors. The setup enables the team to precisely control growth conditions such as oxygen levels.
“This is an important study as they demonstrate ES cells alone can generate whole embryolike structures containing all the early organs completely in vitro,” says cell biologist Jun Wu of the University of Texas Southwestern Medical Center.
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