Nanoparticle drug delivery system super-enhances neuron regeneration in zebrafish eye, new study shows johnshopkins CommsBio
Data were processed using GraphPad Prism 9 to generate all plots as well as carry out statistical analyses recommended by the software based on the type of data generated. For tests involving only two experimental groups , unpaired two-tailedvalues and effect sizes between groups. The majority of assays involved comparisons across multiple groups , and statistical tests done in these cases were unpaired two-tailed Welch’s ANOVAvalues.
To identify differentially expressed genes between conditions, a matrix file containing all Ensembl transcript ID’s and 12 columns, one for each sample, were read into the edgeR Bioconductor package in R/R Studio . Briefly, a model matrix was designed to aggregate data between three biological replicates for each condition. Further analysis of the variance between samples revealed low-quality data for one replicate of Mtz-only RNA compared to the other groups, and thus this group was removed.
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