Live-seq: Sequencing a cell without killing it epfl_en nature
—to manipulate tiny volumes of fluids in a sample under the microscope developed at ETH Zurich some years back. Because of this, FluidFM allows users to insert substances into individual cells, or to extract cytoplasm including mRNA from single cells without having to kill them.
The critical advance leading up to Live-seq emerged when the researchers managed to preserve and read out the mRNA , from these tiny amounts of cytoplasmic sample. As a result, Live-seq can now connect a cell's transcriptome at a given time to its later molecular or phenotypic behavior, i.e. to monitor the activity of thousands of genes in aat discrete time points—what the scientists refer to as a"temporal" transcriptomic analysis.
"With Live-seq, we can now uniquely address highly interesting and biomedically relevant questions, such as why certain cells differentiate and sister cells do not, or why certain cells are resistant to a cancer drug, while their sister cells are again not," says Bart Deplancke. Testing Live-seq, the researchers showed that it can accurately identify diverse cell types and states without introducing major disturbances. As a proof-of-concept, they used their new platform to directly map the"trajectory" of individual immune cells before and after they became active, as well as adipose stromal cells—a type of stem cells—before and after they differentiated into fat cells.
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