This could help in reviving endangered species.
On this occasion, the researchers freeze-dried skin cells from mouse tails and then stored them for up to nine months at -22F before attempting to use them for cloning experiments. The method, however, killed the skin cells, but researchers found that the dead cells could still be used for cloning. Since the DNA or genetic material was still intact in the frozen cells, the researchers transferred it to early-stage mouse embryos whose nuclei were removed.
In all, the researchers obtained 75 cloned mice. Only nine female and three male mice were further allowed to breed with normal mice to determine if the cloned mice were fertile. All females in this experiment had a litter, demonstrating that the method works. The researchers also reported that the method process was inefficient with the success rate of the technique ranging from 0.2 to 5.4 percent. At its lowest, the method fares worse than the one used to clone Dolly, the sheep.
Additionally, in the case of some nuclei, the Y chromosome was badly damaged, resulting in female mice being born from male animal cells. While this could help, if all the females of a species were lost, the method still needs a lot of improvement to be regarded as an importantMaintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen is difficult, expensive, and easily disrupted during disasters.
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