Tuning plant phenotypes by precise, graded downregulation of gene expression
The ability to control gene expression and generate quantitative phenotypic changes is essential for breeding new and desired traits into crops. Here we report an efficient, facile method for downregulating gene expression to predictable, desired levels by engineering upstream open reading frames . We used base editing or prime editing to generate de novo uORFs or to extend existing uORFs by mutating their stop codons.
By combining these approaches, we generated a suite of uORFs that incrementally downregulate the translation of primary open reading frames to 2.5–84.9% of the wild-type level. By editing the 5′ untranslated region of, which encodes a member of the GRAS family and is involved in the brassinosteroid transduction pathway, we obtained, as predicted, a series of rice plants with varied plant heights and tiller numbers.
日本 最新ニュース, 日本 見出し
Similar News:他のニュース ソースから収集した、これに似たニュース記事を読むこともできます。
Women build strength in numbers - Nature BiotechnologyWomen build strength in numbers Women’s networks are strong, and making a difference IWD2023 EmbraceEquity WomenInBiotech
続きを読む »
Faunal engineering stimulates landscape-scale accretion in southeastern US salt marshes - Nature CommunicationsThe contribution of animal ecosystem engineers to coastal geomorphological processes is often neglected. Here, the authors combine observational, experimental and modelling work to demonstrate that ecosystem engineering by mussels is a much stronger driver of salt marsh accretion rates than expected.
続きを読む »
Dynamic crystallography reveals spontaneous anisotropy in cubic GeTe - Nature MaterialsCubic materials such as GeTe have low lattice thermal conductivity, thought to arise from a non-cubic local structural transition. Here, using a variable-shutter pair distribution function method, the average structure is shown to be crystalline but with anisotropic dynamics at higher temperatures.
続きを読む »
Evolution of synchronous female bilateral breast cancers and response to treatment - Nature MedicineClinical, genomic and transcriptomic analyses of paired samples of synchronous bilateral female breastcancer identify associations between tumor concordance and immune infiltrates levels and response to neoadjuvant treatment FabienReyal JoshWaterfall
続きを読む »
An elegant portrait of a pole dancer in Oman celebrates a woman's strength in natureEman Ali's arresting image of Nusaiba Al Maskari is set against the mountainous backdrop of Muscat, where pole dancing is rarely practiced.
続きを読む »