Nature research paper: Structural mechanisms for regulation of GSDMB pore-forming activity
, Purification of GSDMB pores by gel-filtration chromatography. The highlighted fraction containing homogenous pores were analyzed by SDS-PAGE and also applied to negative-stain EM examination and final cryo-EM data collection. Negative-stain EM image of GSDMB pores is shown. Scale bar, 100 nm., Percentages of GSDMB pores with C26, C27, C28, C29 and C30-fold symmetry among a total of 270,000 top-view pore particles.pores on the liposomes were measured.
Extended Data Fig. 8 Structural and functional analyses of GSDMB isoforms and their relevance to cancer cell pyroptosis., Close-up view of structures around exon-6 region in the GSDMB-N domain. GSDMB-N structures in the autoinhibited state and in the pore are overlaid, with exon-6 regions highlighted in wheat. Relevant structural elements are labeled and crucial residues are in sticks.
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