How pancreaticcancer defies treatment by leveraging a protein ucsandiego NatureComms
cells were infected with RFP-tagged shRNA againstor control in triplicate; transduced cells were sorted 72 h post-transduction and plated in a 10 cm plate in 10% DMEM growth media. 5 days later, cells were collected for analysis by RNA-seq and ChIP-seq. >300,00 cells per replicate were collected for RNA-seq; total RNA was isolated using Quick-RNA Miniprep Kit . RNA was assessed for quality using an Agilent Tapestation; all samples had RIN > 7.
was used to process alignment files to generate ChIP-seq bed files. ChIP-seq peaks for with the parameter “-style factor” versus the appropriate ChIP input experiments as background. ChIP-seq peaks for H3K4me, H3K4me3, and H3K27ac were called using the parameter “-style histone”. database using the findMotifsGenome.pl command using default fragment size and motif length parameters. Random GC content-matched genomic regions were used as background. Enriched motifs are statistically significant motifs in input over background by aFor RNA-seq, RNA expression was quantified as raw integer counts using analyzeRepeats.pl in
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Ultrasensitive rapid cytokine sensors based on asymmetric geometry two-dimensional MoS2 diodes - Nature CommunicationsDetection of cytokine biomarkers has the potential to aid in diagnosis and treatment of different diseases. Here, the authors report on the creation of an asymmetric geometry MoS2 diode-based biosensor for the detection of TNF-α as a model biomarker in a proof of concept study.
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What does the future of clinical trials and evidence-based medicine look like?In a recent perspective published in Nature Medicine, the researcher shared his vision and future perspectives for the domains of clinical research and evidence-based therapeutics.
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Integration of Hi-C with short and long-read genome sequencing reveals the structure of germline rearranged genomes - Nature CommunicationsHere the authors characterize structural variations (SVs) in a cohort of individuals with complex genomic rearrangements, identifying breakpoints by employing short- and long-read genome sequencing and investigate their impact on gene expression and the three-dimensional chromatin architecture. They find breakpoints are enriched in inactive regions and can result in chromatin domain fusions.
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Footprints in snow help Preston police identify suspectsOfficers investigating an offence thank 'Mother Nature for her assistance' in catching two suspects.
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I’ve been skinny-shamed and fat-shamed… I know which hurts the most“I want to talk about why people think it’s okay to tell slim people they need to put weight on.'
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