Researchers discover a sustainable way to repurpose tart cherry waste, harnessing its bioactive compounds for skin care applications. The study reveals that these extracts significantly protect human skin cells from oxidative stress, offering an eco-friendly solution to both food waste and skin health.
By Hugo Francisco de SouzaOct 16 2023Reviewed by Benedette Cuffari, M.Sc. In a recent study published in Foods, researchers investigate environmentally friendly and sustainable uses for processed tart cherry waste. Their study focused on cosmetic applications, including optimizing the extraction of bioactive metabolites from cherries and their beneficial impacts on human skin.
Over 90% of cherries are processed by the food industry to produce juice, brined products, and wine. This leads to the generation of byproducts, including cherry pomace and cherry pits , almost all of which are burned or discarded. Polyphenols, one of the largest classes of plant-derived metabolites, have been shown to exert antioxidant activity in vitro, potentially reducing skin inflammation. Cherries are rich in polyphenols and, as a result, could be a source of novel cosmetic products. Although previous studies have investigated the benefits of cherry byproducts from the stems and seeds, the antioxidant activity of CP still needs to be evaluated.
A double-beam Lambda 365 UV-Vis spectrophotometer was used to quantify the polyphenol , antiradical powder , and flavonoid content of the supernatant. For increased accuracy of TP quantification, the colorimetric Folin-Ciocalteu assay was employed. Simultaneously, a second colorimetric assay was performed to investigate the in vitro antioxidant activity of TP, TF, and ARP against 2,2-azinobis .
Intracellular ROS activity was measured using fluorescence microscopy. RNA isolation and real-time-polymerase chain reaction analyses were also performed to identify HaCaT and NIH3T3 genes that were up- or down-regulated following extract treatment. LC-MS/MS results identified and quantified multiple caffeoylquinate ester isomers, hydroxycinnamic acids, and flavonoids. CPE extracts depicted higher concentrations of compounds than CPW. Lyophilized CP extracts similarly exhibited higher concentrations of bioactives than liquid extracts, thus suggesting future industrial optimizations in extraction protocols.
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