Study indicates likely cause of common penis birth-defect SciReports
. Similar studies with hypospadias are required in the future and will provide further insights into environmentally induced hypospadias etiology.
Combined observations identify a better understanding of the etiology of hypospadias on a molecular level so as to integrate environmental impacts and clarify the severity of hypospadias progression.
. Foreskin tissues were after collection stored at − 20 °C until shipment and then stored at − 80 °C.Frozen human foreskin tissue samples were stored at − 80 °C and thawed for analysis. Genomic DNA from foreskin tissue samples was prepared as follows: the foreskin tissue was suspended in 750 μl of cell lysis solution and 3.5 µl of Proteinase K . This suspension was incubated at 55 °C for 3 h, then vortexed and centrifuged briefly.
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