Researchers report the circulation of a novel MERS-like coronavirus in Malayan pangolins CellPressNews coronavirus virus virology zoonoticdisease infectiousdisease
By Pooja Toshniwal PahariaFeb 21 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in Cell, researchers reported the circulation of a novel Middle East respiratory syndrome -like coronavirus among Malayan pangolins, Manis javanica HKU4-associated CoV .
About the study In the present study, researchers explored pangolins as reservoirs or susceptible host species for zoonotic bat CoV transmission. To investigate MjHKU4r-CoV prevalence among pangolins, MjHKU4-CoV-1 nucleocapsid protein-based luciferase immunoprecipitation system assays were performed. Further, viral particles were examined by transmission electron microscopy, and immunofluorescence analysis was performed using human colorectal adenocarcinoma cells for NP staining.
Results Antibodies eBook Compilation of the top interviews, articles, and news in the last year. Download a free copy A novel MERS-like coronavirus MjHKU4r-CoV, phylogenetically associated with bat HKU4- CoV was identified among Malayan pangolins. The isolated MjHKU4r-CoV-1 virus Caco-2 cells used hDPP4 and cellular proteases for cell entry, and the binding was enhanced by a furin cleavage site absent in known bat HKU4r-CoVs.
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