How post-feeding breasts bounce back from the brink of death to kick-start milk production sheffielduni PLOSBiology
]. Data accession: E-MTAB-5019 or GSE85188 . Gene lists were analysed using DAVID, Panther, and GSEA web accessible programs.Quantitative RT-PCR was performed on the Applied Biosystems 7900HT using the following Taqman probes : Krt18 , MAPKI as housekeeping genes, CCL2 , CCL7 , Csn2 . Thermal cycling conditions were as follows: UNG start at 50°C , 95°C , and then 40 cycles of 95°C followed by annealing at 60°C .Primary MECs were harvested from either 15.5 to 17.
Primary mammary cells embedded in a BM-matrix were cultured with either unconditioned media or conditioned media from M0 or M2 macrophages. Organoids were treated with 10 μM EdU for 2 h prior to processing.Apoptotic MECs were prepared in suspension culture for either 1 h or 5 h, in serum-free media .
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