Mitochondrial genome editing technique yields useful traits

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Mitochondrial genome editing technique yields useful traits
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Tweaks to the mitochondrial genome hold the potential for better hybrid seed production or to introduce seedless fruits.

North Carolina State University researchers have successfully transferred an important gene from one compartment of a plant cell to another to produce tobacco plants that lack pollen and viable seeds, while otherwise growing normally. Their findings could lead to better ways of producing hybrid seeds to maximize crop productivity, or to introduce seedlessness in fruit species lacking the often-desired trait, such as raspberries, blackberries or muscadine grapes.

In their proof-of-concept study, the NC State researchers, together with colleagues from Precision BioSciences and Elo Life Systems, deployed a unique strategy to test whether the CMS trait could be generated in tobacco, a commonly used model species in plant research.

"That's because the promoter that we chose not only failed to express during pollen formation, but also during early seed development," Dewey said.Dewey said that his team is now working to decouple these results so that researchers can achieve either pollen infertility or the seedless trait alone, instead of both at the same time.

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