Researchers unveil the complex cellular architecture of the heart using advanced RNA sequencing and imaging techniques, shedding light on the intricate organization of heart cells and their developmental pathways.
By Dr. Chinta SidharthanMar 14 2024Reviewed by Susha Cheriyedath, M.Sc. In a recent study published in the journal Nature, a large team of researchers from the United States used single-cell ribonucleic acid sequencing combined with high-resolution fluorescence in situ hybridization to determine the identities of the various cell types that coordinate spatially to give rise to the complex morphological structure of the heart.
About the study In the present study, the researchers used a single-cell RNA sequencing approach along with multiplexed error-robust fluorescence in situ hybridization . This strategy allowed them to combine single-cell transcriptomes and spatial biology and visualize, analyze, and quantify the RNA transcripts of a large number of genes from a single cell.
MER-FISH was then used to spatially map the heart cells and explore the cellular mechanisms through which the remodeling and morphogenesis of the heart, including the ventricular wall development, were directed. The organization of the cells identified using scRNAseq, especially during developmental periods such as the compaction of the myocardial wall, was explored using MER-FISH imaging.
Results The findings revealed that various cardiac cell types belonged to specific subpopulations that were part of specific communities, with the functional specialization defined according to the anatomical region in which they were present and the cellular ecosystem. The cardiomyocyte lineages were the largest cell compartment identified using MER-FISH.
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