Novel method facilitates geneticstudies of the function and diseases of the intestinalepithelium
Together, this study demonstrates a new microinjection technique that enables robust, reproducible, and stable in vivo targeting of intestinal stem cells in situ in the unperturbed intestinal epithelium. The technique enables researchers to genetically target ISCs and the intestinal epithelium in a physiological in vivo setting. The new technique is capable of transducing all regions of the gastrointestinal tract including stomach, small intestine, and colon.
All animals were housed in centralized facilities at the Fred Hutchinson Cancer Center under the care and supervision of the Comparative Medicine Unit . The animal facility is managed in compliance with the Guide for the Care and Use of Laboratory Animals, 2011. The FHCC is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care, International assured by NIH Office of Animal Welfare and registered as a research facility with the USDA.
Once pulled, the needle tip was snapped off using fine tip forceps at the level where its diameter was30 μm. The needle tip was bevelled at 25° on a fine-grade abrasive plate with regular wetting for 10 min. Afterwards, the needle tip was microscopically checked under × 10 magnification to ensure a clean bevel without deformities.
日本 最新ニュース, 日本 見出し
Similar News:他のニュース ソースから収集した、これに似たニュース記事を読むこともできます。
BMC Ecology and Evolution Image Competition 2023 - BMC Series blogWe are pleased to announce the launch of our third BMC Ecology and Evolution image competition! Submit your images and the stories behind them for a chance to highlight your research, win prizes, and have your photography featured in the journal.
続きを読む »
BMC Global and Public HealthBMC Global and Public Health is pleased to welcome CFMcQuaid to its Editorial Board. Finn is an infectious disease epidemiologist working on TB modelling at LSHTM. He is also co-guest editor for a collection on identifying people with TB.
続きを読む »
Lifestyle management in polycystic ovary syndrome – beyond diet and physical activity - BMC Endocrine DisordersPolycystic ovary syndrome (PCOS) is a common condition affecting reproductive-aged women with reproductive, metabolic and psychological consequences. Weight and lifestyle (diet, physical activity and behavioural) management are first-line therapy in international evidence-based guidelines for PCOS. While these recommend following population-level diet and physical activity guidelines, there is ongoing interest and research in the potential benefit of including psychological and sleep interventions, as well as a range of traditional, complimentary and integrative medicine (TCIM) approaches, for optimal management of PCOS. There is limited evidence to recommend a specific diet composition for PCOS with approaches including modifying protein, carbohydrate or fat quality or quantity generally having similar effects on the presentations of PCOS. With regards to physical activity, promising evidence supports the provision of vigorous aerobic exercise, which has been shown to improve body composition, cardiorespiratory fitness and insulin resistance. Psychological and sleep interventions are also important considerations, with women displaying poor emotional wellbeing and higher rates of clinical and subclinical sleep disturbance, potentially limiting their ability to make positive lifestyle change. While optimising sleep and emotional wellbeing may aid symptom management in PCOS, research exploring the efficacy of clinical interventions is lacking. Uptake of TCIM approaches, in particular supplement and herbal medicine use, by women with PCOS is growing. However, there is currently insufficient evidence to support integration into routine clinical practice. Research investigating inositol supplementation have produced the most promising findings, showing improved metabolic profiles and reduced hyperandrogenism. Findings for other supplements, herbal medicines, acupuncture and yoga is so far inconsistent, and to reduce heterogeneity more research in specific PCOS populatio
続きを読む »
BMC Global and Public HealthBMC Global and Public Health is pleased to welcome DaftaryAmrita to its Editorial Board. She is a social and behavioural health researcher investigating the social determinants of tuberculosis and HIV/AIDS YUGlobalHealth.
続きを読む »
Polypharmacy during pregnancy and associated risk factors: a retrospective analysis of 577 medication exposures among 1.5 million pregnancies in the UK, 2000-2019 - BMC MedicineBackground The number of medications prescribed during pregnancy has increased over the past few decades. Few studies have described the prevalence of multiple medication use among pregnant women. This study aims to describe the overall prevalence over the last two decades among all pregnant women and those with multimorbidity and to identify risk factors for polypharmacy in pregnancy. Methods A retrospective cohort study was conducted between 2000 and 2019 using the Clinical Practice Research Datalink (CPRD) pregnancy register. Prescription records for 577 medication categories were obtained. Prevalence estimates for polypharmacy (ranging from 2+ to 11+ medications) were presented along with the medications commonly prescribed individually and in pairs during the first trimester and the entire pregnancy period. Logistic regression models were performed to identify risk factors for polypharmacy. Results During the first trimester (812,354 pregnancies), the prevalence of polypharmacy ranged from 24.6% (2+ medications) to 0.1% (11+ medications). During the entire pregnancy period (774,247 pregnancies), the prevalence ranged from 58.7 to 1.4%. Broad-spectrum penicillin (6.6%), compound analgesics (4.5%) and treatment of candidiasis (4.3%) were commonly prescribed. Pairs of medication prescribed to manage different long-term conditions commonly included selective beta 2 agonists or selective serotonin re-uptake inhibitors (SSRIs). Risk factors for being prescribed 2+ medications during the first trimester of pregnancy include being overweight or obese [aOR: 1.16 (1.14–1.18) and 1.55 (1.53–1.57)], belonging to an ethnic minority group [aOR: 2.40 (2.33–2.47), 1.71 (1.65–1.76), 1.41 (1.35–1.47) and 1.39 (1.30–1.49) among women from South Asian, Black, other and mixed ethnicities compared to white women] and smoking or previously smoking [aOR: 1.19 (1.18–1.20) and 1.05 (1.03–1.06)]. Higher and lower age, higher gravidity, increasing number of comorbidities and increasing le
続きを読む »
Cortical Grey matter volume depletion links to neurological sequelae in post COVID-19 “long haulers” - BMC NeurologyObjective COVID-19 (SARS-CoV-2) has been associated with neurological sequelae even in those patients with mild respiratory symptoms. Patients experiencing cognitive symptoms such as “brain fog” and other neurologic sequelae for 8 or more weeks define “long haulers”. There is limited information regarding damage to grey matter (GM) structures occurring in COVID-19 “long haulers”. Advanced imaging techniques can quantify brain volume depletions related to COVID-19 infection which is important as conventional Brain MRI often fails to identify disease correlates. 3-dimensional voxel-based morphometry (3D VBM) analyzes, segments and quantifies key brain volumes allowing comparisons between COVID-19 “long haulers” and normative data drawn from healthy controls, with values based on percentages of intracranial volume. Methods This is a retrospective single center study which analyzed 24 consecutive COVID-19 infected patients with long term neurologic symptoms. Each patient underwent Brain MRI with 3D VBM at median time of 85 days following laboratory confirmation. All patients had relatively mild respiratory symptoms not requiring oxygen supplementation, hospitalization, or assisted ventilation. 3D VBM was obtained for whole brain and forebrain parenchyma, cortical grey matter (CGM), hippocampus, and thalamus. Results The results demonstrate a statistically significant depletion of CGM volume in 24 COVID-19 infected patients. Reduced CGM volume likely influences their long term neurological sequelae and may impair post COVID-19 patient’s quality of life and productivity. Conclusion This study contributes to understanding effects of COVID-19 infection on patient’s neurocognitive and neurological function, with potential for producing serious long term personal and economic consequences, and ongoing challenges to public health systems.
続きを読む »