Researchers uncover insights into the evolution of color patterns in frogs and toads
species complex were collected in the highlands of Ethiopia between 2011 and 2019. Our study was approved by the relevant Institutional Animal Care and Use Committee at Queens College and New York University School of Medicine . Frogs were sampled according to permits DA31/305/05, DA5/442/13, DA31/454/07, DA31/192/2010, DA31/230/2010, DA31/7/2011, and DA31/02/11 provided by the Ethiopian Wildlife Conservation Authority.
). Briefly, a de novo assembly was first constructed using two paired end libraries of mean insert size ∼350 bp and one paired end library of ∼550 bp insert size. The shotgun reads were incorporated with low-coverage PacBio to generate a hybrid assembly. To achieve longer scaffolds, two Chicago libraries were sequenced as described in
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