The function of single-nucleotide variants can now be tested in a base editor mouse model.
on normal chow or maintained on doxycycline chow for 14 days . Data are presented as mean values ± s.d for both sgRNAs.. Sequencing analysis at cancer gene sites in cell conditions described in a. Solid blue boxes represent on-target activity of the sgRNA, dotted orange boxes signify on-target ‘bystander’ editing within the gRNA window.. Quantification of C>T and C>other SNVs found across both targets.
2-way ANOVA test for multiple comparisons was used to evaluate statistical significance across conditions. Data are presented as mean values ± s.e.m. p-values are displayed.. Schematic of experimental set up in iBE derived pancreatic organoids. Organoids were transduced and selected with GFPreporter . Organoids maintained off dox were then split into dox conditions to induce BE expression for 4 days and then split again into + and – dox conditions for an additional day..
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