A dozen exotic bacteria are found to passively collect rareearthelements from wastewater frontiersin
gas. Biomass was harvested by centrifugation after reaching the stationary phase.2.2 Phylogenetic characterization of strains
About 50 mg of biomass from all cultures were collected during stationary growth phase and used for gDNA extraction with the DNeasy PowerSoil Pro Kit following the manufacturer’s instructions. The 16S–23S ITS gene region was amplified by PCR in a 50 µL reaction using the primers Wil1 and Wil18 and ready-to-go PCR mini beads in a MiniAmp Plus Thermal Cycler . PCR products were checked by gel electrophoresis using 1% agarose and the E-Gel Power Snap Electrophoresis System .
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