The route between Edinburgh and Fife will take passengers over the Forth Road Bridge from 15 May.
They will have two members of staff on board.
The UK government said Project CAVForth would be the world's first full-size, self-driving public bus service.Scottish government Transport Minister Kevin Stewart said: "This is an exciting milestone for this innovative and ambitious project, and I very much look forward to seeing Project CAVForth take to the roads next month.
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