Discovery of 16th century coronavirus in France Coronavirus France dental paleoserology paleoproteomic SARSCoV2229E ISID_org univamu UnivParisSaclay
By Dr. Chinta SidharthanMar 20 2023Reviewed by Danielle Ellis, B.Sc. They uncovered amino acid evidence of an ancient betacoronavirus from the 16th century.
Coronaviruses have been detected in upper respiratory tract cell culture samples from the 1960s, and several studies have uncovered human-associated alpha and beta-coronaviruses and animal-associated coronaviruses that share a similar ribonucleic acid genome containing genes to encode nucleocapsid and spike proteins. Anti-coronavirus antibodies have also been detected in five soldiers who died in France during the first world war in 1914.
Cell lines such as Vero E6, HCT-8, and MRCC-5 were inoculated with betacoronaviruses SARS-CoV-2 and HCoV-OC43 and alphacoronavirus to produce the specific antigens, which were further verified through reverse-transcription polymerase-chain-reaction and Western immunoblotting. These antigens, along with skim milk as a negative control and antigen from Staphylococcus aureus as a positive control, were used for the blot assays.
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