Nature research paper: ADAR1 averts fatal type I interferon induction by ZBP1
−/−
mice, two sgRNAs , each at 4 µM, in complex with 4 µM Cas9 protein were co-electroporated into fertilized oocytes. Deletion of theexon between the two sgRNAs was confirmed using primers 5′-ATCCCACTAGAACAACCCTGCC-3′ and 5′-TTCAGACTCCGCACCCTCATTT-3′ as well as by immunoblot analysis. Formice, two sgRNAs targeting exons 3 and 6, respectively, were co-injected into fertilized C57BL/6N oocytes at 50 ng µlCas9 mRNA .
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