New, portable device detects glow emitted by plants to measure their health
) were used in measurements, with five signal averaged measurements per species. Multiple heat and chill stress experiments were conducted with readily available and well-studiedto ensure similar trends in delayed fluorescence were observed. All photon counting studies were performed within one hour of cutting the leaf from the plant with the exception of the spinach leaf.
As with cold-shock, we similarly exposed the plant leaves to acute heat stress and examined the time-dependent delayed fluorescence behavior with our photon counting SiPM biosensor upon heating a leaf from ambient conditions overtop a light-tight thermally regulated hotplate at 40 °C and 50 °C for 15 min. After both heat and chill shock stressors, the leaf exposed to these extreme temperatures was returned to 21 °C ambient conditions to examine delayed fluorescence recovery.
. The device’s footprint is 30 cm × 23 cm. The system uses an ON Semiconductor model 10035 silicon photomultiplier . This is a 1 mmelectrons per photon. ON Semiconductor SiPMs incorporate a “fast output”, a capacitively coupled output taken directly off each SPAD’s quench resistor, resulting in sub-nanosecond spikes on the order of a few hundred microvolts.
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